Search results for " biopreservation"

showing 3 items of 3 documents

Proteins in Saccharides Matrices and the Trehalose Peculiarity: Biochemical and Biophysical Properties

2015

Immobilization of proteins and other biomolecules in saccharide matrices leads to a series of peculiar properties that are relevant from the point of view of both biochemistry and biophysics, and have important implications on related fields such as food industry, pharmaceutics, and medicine. In the last years, the properties of biomolecules embedded into glassy matrices and/or highly concentrated solutions of saccharides have been thoroughly investigated, at the molecular level, through in vivo, in vitro, and in silico studies. These systems show an outstanding ability to protect biostructures against stress conditions; various mechanisms appear to be at the basis of such bioprotection, th…

Organic Chemistrytrehalose protein dynamics biopreservation myoglobinSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)Current Organic Chemistry
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SAXS Study on Myoglobin Embedded in Amorphous Saccharide Matrices

2011

We report on Small Angle X-ray Scattering (SAXS) measurements performed on samples of carboxy-myoglobin and met-myoglobin embedded in low hydrated matrices of four different saccharides (trehalose, sucrose, maltose and lactose). Results confirm the already reported occurrence of inhomogeneities, which are not peculiar of trehalose samples, but appear also in maltose and lactose, and in some cases also sucrose, being dependent on the sample hydration and on the presence of sodium dithionite. This behaviour confirms our previous interpretation about the nature of the inhomogeneities, and prompt it as a possible general behaviour for highly concentrated sugar matrices.

Small-angle X-ray scatteringMyoglobinBiophysicsAnalytical chemistrySurfaces and InterfacesGeneral ChemistryMaltoseDisaccharidesTrehaloseSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)Sodium dithionitechemistry.chemical_compoundchemistryMyoglobinX-Ray Diffractionmyoglobin saccharide sugar trehalose sucrose maltose lactose bioprotection biopreservation SAXS Small-Angle X-Ray ScatteringScattering Small AngleAnimalsGeneral Materials ScienceSoft matterLactoseSugarMetmyoglobinBiotechnology
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FTIR Study on Reciprocal Protein↔Matrix effects in dry amorphous saccharide systems

2009

trehalose saccharide carboxymyoglobin FTIR biopreservation
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